ВЛИЯНИЕ МЕДИ НА КОМПОНЕНТЫ АНТИОКСИДАНТНОЙ СИСТЕМЫ ПИЩЕВАРИТЕЛЬНОЙ ЖЕЛЕЗЫ ПРЕСНОВОДНОГО ДВУСТВОРЧАТОГО МОЛЛЮСКА ANODONTA CYGNEA

Ирина Викторовна Суховская, Станислав Ремасо Курпе, Екатерина Витальевна Борвинская, Альбина Александровна Кочнева, Наталья Николаевна Фокина, Irina Sukhovskaya, Stanislav Remaso Kurpe, Ekaterina Borvinskaya, Albina Kochneva, Natalia Fokina

Аннотация


Исследованы эффекты аккумуляции меди и ее воздействия на работу некоторых компонентов системы антиоксидантной защиты (АОС) (САТ, GST, GSH) в пищеварительной железе пресноводного двустворчатого моллюска Anodontacygneaв условиях аквариального эксперимента. После 12-ти дневной акклимации моллюсков рассадили по аквариумам с концентрацией Cu2+ 0, 5, 50, 100 и 250 мкг/л. Образцы тканей гепатопанкреаса для анализа отбирали через 1, 3 и 7 суток. В ткани определяли содержание ионов меди, активность GST и САТ, а также концентрацию GSH. Показано, что процесс накопления носит дозо- и времязависимый эффект. Аккумулированный в данной концентрации металл не привел к гибели животных. Скорость накопления металла в исследованном органе увеличивалась с повышением концентрации ионов меди в воде. Воздействие повышенных концентраций меди приводит к адаптивному изменению активности ферментов АОС у беззубки, направленному на обезвреживание продуктов окислительных реакций. Изменения в активности ферментов АОС (САТ и GST) были отмечены, в основном, на седьмые сутки эксперимента при самых высоких из протестированных концентраций меди. GST проявила себя наиболее стабильным ферментом. Уровень GSH в пищеварительной железе менялся через 1 сутки от начала эксперимента, подтверждая участие этого трипептида в первом эшелоне защиты от ксенобиотиков. Ионы меди обладают кумулятивным эффектом. Отмеченные на 7 сутки изменения активности ферментов при концентрации Cu2+ 250 мкг/л позволяют предположить, что более длительное действие меди, даже в незначительных концентрациях может приводить к негативным последствиям.


Ключевые слова


оксидативный стресс; система антиоксидантной защиты; адаптация; аккумуляция металла; медь

Полный текст:

PDF

Литература


Государственный доклад «О состоянии и использовании водных ресурсов Российской Федерации в 2017 году». М.: НИА-Природа, 2018. 298 с.

Даувальтер В. А., Кашулин Н. А. Изменение концентраций никеля и меди в поверхностных слоях донных отложений оз. Имандра за последние полвека // Вестник МГТУ. 2015. Т. 18, № 2. С. 307–321.

Лукьянова О. Н. Молекулярные биомаркеры. Владивосток: ДВГАЭУ, 2001. 196 с.

Мишукова Т. Г., Осипов А. А., Сальников И. А. Определение содержания микроэлементов в питьевых водах Оренбургской области // Вестник Оренбургского гос. университета. 2015. Т. 10, № 185. С. 303–307

Моисеенко Т. И., Кудрявцева Л. Н., Гашкина Н. А. Рассеянные элементы в поверхностных водах суши: технофильность, биоаккумуляция и экотоксикология. М.: Наука, 2006. 261 с.

Озера Карелии: Справочник / Ред. Н. Н. Филатов, В. И. Кухарев. Петрозаводск: КарНЦ РАН, 2013. 464 с.

Приказ от 13 декабря 2016 года № 552 «Об утверждении нормативов качества воды водных объектов рыбохозяйственного значения, в том числе нормативов предельно допустимых концентраций вредных веществ в водах водных объектов рыбохозяйственного значения (с изменениями на 12 октября 2018 года)» Министерства сельского хозяйства Российской Федерации // Электронный фонд правовой и нормативно-технической документации

[Электронный ресурс]. URL: http://docs.cntd.ru/document/

(дата обращения 15.11.2019).

Слуковский З. И. Нормирование по литию концентраций тяжелых металлов в донных отложениях озер Ладожское и Четырехверстное (Республика Карелия) // Химия в интересах устойчивого развития. 2015. Т. 23, № 4. С. 397–408. doi: 10.15372/KhUR20150409

Слуковский З. И., Полякова Т. Н. Анализ накопления тяжелых металлов в организме олигохет из речных донных отложений урбанизированной среды // Биология внутренних вод. 2017. № 3. C. 73–82. doi: 10.7868/S032096521703010X

Суховская И. В., Борвинская Е. В., Смирнов Л. П., Немова Н. Н. Сравнительный анализ методов определения концентрации белка – спектрофотометрии в диапазоне 200–220 нм и по Брэдфорд // Труды КарНЦ РАН. 2010. № 2. С. 68–71.

Чуйко Г. М. Биомаркеры в гидроэкотоксикологии: принципы, методы и методология, практика использования // Экологический мониторинг. Ч. VIII. Современные проблемы мониторинга пресноводных экосистем: учебное пособие / Ред. Д. Б. Гелашвили, Г. В. Шурганова. Нижний Новгород: ННГУ, 2014. С. 309–326.

Шилова Н. А. Влияние тяжелых металлов на представителей пресноводного фито- и зоопланктона в условиях засоления: Автореф. дис. … канд. биол. наук. Саратов, 2014. 133 с.

Ahsanullah M., Williams A. R. Sublethal effects and bioaccumulation of cadmium, chromium, copper and zinc in the marine amphipod Allorchestes compressa // Mar. Biol. 1991. Vol. 108. P. 59–65. doi: 10.1007/BF01313471

Beers R. F. Jr., Sizer I. W. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase // Biol. Chem. 1952. Vol. 195, no. 1. P. 133–140.

Cajaraville M. P., Bebianno M. J., Blasco J., Porte C., Sarasquete C., Viarengo A. The use of biomarkers to assess

the impact of pollution in coastal environments of the Iberian Peninsula: a practical approach // Sci. Total. Environ. 2000. Vol. 247, no. 2–3. P. 295–311. doi: 10.1016/s0048-9697(99)00499-4

Canesi L., Ciacci C., Fabbri R., Marcomini A., Pojana G., Gallo G. Bivalve mollusks as a unique target group for nanoparticle toxicity // Mar. Environ. Res. 2012. Vol. 76. P. 16–21. doi: 10.1016/j.marenvres.2011.06.005

Canesi L., Malatesta M., Battistelli S., Gallo G., Gazzanelli G. Identification of epidermal growth factor receptors by immunoelectron microscopy in isolated mussel digestive gland cells // Abstracts 19th Conference of European Comparative Endocrinologists (Nijmegen, The Netherlands, September 1–5, 1998).

Chapman P. M. Environmental risks of inorganic metals and metalloids: A continuing, evolving scientific odyssey // Human Ecol. Risk Assess. 2008. Vol. 14, no. 1. P. 5–40. doi: 10.1080/10807030701790272

Conradi M., Depledge M. H. Population responses of the marine amphipod Corophiumvolutator (Pallas, 1766) to copper // Aquat. Toxicol. 1998. Vol. 44. P. 31–45. doi: 10.1016/S0166-445X(98)00069-1

Cosson R. P., Thiébaut E., Company R., Castrec-Rouelle M., Colaço A., Martins I., Sarradin P. M., Bebianno M. J. Spatial variation of metal bioaccumulation in the hydrothermal vent mussel Bathymodiolus azoricus // Mar. Environ. Res. 2008. Vol. 65, no. 5. P. 405–415. doi: 10.1016/j.marenvres.2008.01.005

Directive 2008/105/EC of the European Parliament and of the Council of 16 December 2008 on environmental quality standards in the field of water policy, amending and subsequently repealing Council Directives 82/176/EEC, 83/513/EEC, 84/156/EEC, 84/491/EEC, 86/280/EEC and amending Directive 2000/60/EC of the European Parliament and of the Council // Official J. of the European Union. 2008. Vol. L 348. P. 84–97.

Dondero F., Piacentini L., Banni M., Rebelo M., Burlando B., Viarengo A. Quantitative PCR analysis of two molluscan metallothionein genes unveils differential expression and regulation // Gene. 2005. Vol. 345, no. 2. P. 259–270. doi: 10.1016/j.gene.2004.11.031

Duffus J. H. “Heavy metals” a meaningless term? (IUPAC Technical Report) // Pure Appl. Chem. 2002. Vol. 74, no. 5. P. 793–807.

Foster-Smith R. L. The effect of concentration of suspension of the filtration rates and pseudofaecal production for Mytilus edulis L., Cerastoderma edule (L.) and Venerupis pullastra (Montagu) // J. Exp. Mar. Biol. Ecol. 1975. Vol. 17. P. 1–22. doi: 10.1016/0022-0981(75)90075

George S. G., Coombs T. L., Pirie B. J. S. Characterization of metal-containing granules from the kidney of the common mussel, Mytilus edulis // Biochim. Biophys. Acta (BBA) – General Subjects. 1982. Vol. 716, no. 1. P. 61–71. doi: 10.1016/0304–4165(82) 90203–3

George S. G., Pirie B. J. S., Coombs T. L. The kinetics of accumulation and excretion of ferric hydroxide in Mytilus edulis (L.) and its distribution in the tissues // J. Exp. Mar. Biol. Ecol. 1976. Vol. 23, no. 1. P. 71–84. doi: 10.1016/0022-0981(76)90086-1

Geret F., Serafim A., Barreira L., Bebianno M. J. Effects of cadmium on anti-oxidant enzyme activities and lipid peroxidation in the gills of the clam, Ruditapes decussates // Biomarkers. 2002. Vol. 7, no. 3. P. 242–256.

Guéguen M., Amiard J. C., Arnich N., Badot P. M., Claisse D., Guérin T., Vernoux J. P. Shellfish and residual chemical contaminants: hazards, monitoring, and health risk assessment along French coasts // Rev. Environ. Contam. Toxicol. 2011. Vol. 213. P. 55–111. doi: 10.1007/978‑1‑4419‑9860‑6_3

Habig W. H., Pabst M. J., Jakoby W. B. Glutathione S-Transferases. The first enzymatic step in mercapturic acid formation // J. Biol. Chem. 1974. Vol. 249, no. 22. P. 7130–7139.

Hissin P. J., Hilf R. A fluorometric method for determination of oxidized and reduced glutathione in tissues // Anal. Biochem. 1976. Vol. 74, no. 1. P. 214–226.

Hoarau P., Damiens G., Roméo M., Gnassia-Barelli M., Bebianno M. J. Cloningand expression of a GSTpi gene in Mytilus galloprovincialis. Attempt to use the GST-pi transcript as a biomarker of pollution // Comp. Biochem. Physiol. 2006. Vol. 143. P. 196–203.

Huggett R. J., Kimerle R. A., Mehrle P. M., Bergman H. L. Biomarkers: Biochemical, physiological, and histological markers of anthropogenic stress. FL, Boca Raton: Lewis Publ., 1992.

Klaassen C. D., Liu J., Choudhuri S. Metallothionein: An intracellular protein to protect against cadmium toxicity // Annu. Rev. Pharmacol. Toxicol. 1999. Vol. 39. P. 267–294.

Larsen B. K., Pörtner H. O., Jensen F. B. Extraand intracellular acid-base balance and ionic regulation in cod (Gadus morhua) during combined and isolated exposures to hypercapnia and copper // Mar. Biol. 1997. Vol. 128. P. 337–346. doi: 10.1007/s002270050099

Lyons B. P., Thain J. E., Stentiford G. D., Hylland K., Davies I. M., Vethaak A. D. Using biological effects tools to define Good Environmental Status under the European Union Marine Strategy Framework Directive // Mar. Pollut. Bull. 2010. Vol. 60, no. 10. P. 1647–1651. doi: 10.1016/j.marpolbul.2010.06.005

Lysenko L., Sukhovskaya I., Borvinskaya E., Krupnova M., Kantserova N., Bakhmet I., Nemova N. Detoxification and protein quality control markers in the mussel Mytilus edulis (Linnaeus) exposed to crude oil: Salinity-induced modulation // Estuar. Coast. Shelf Sci. 2015. Vol. 167. P. 220–227.

March F. A., Dwyer F. J., Augspurger T., Ingersoll C. G., Wang N., Mebane C. A. An evaluation of freshwater mussel toxicity data in the derivation of water quality guidance and standards for copper // Environ. Toxicol. Chem. 2007. Vol. 26. P. 2066–2074. doi: 10.1897/06-560R. 1

Marigomez I., Soto M., Cajaraville M. P., Angulo E., Giamberini L. Cellular and subcellular distribution of metals in molluscs // Microsc. Res. Tech. 2002. Vol. 56. P. 358–392. doi: 10.1002/jemt.10040

Noble J. E., Bailey M. A. Quantitation of protein // Methods Enzymol. 2009. Vol. 463. P. 73–95. doi: 10.1016/S0076-6879(09)63008-1

Parry H. E., Pipe R. K. Interactive effects of temperature and copper on immunocompetence and disease susceptibility in mussels (Mytilus edulis) // Aquat. Toxicol. 2004. Vol. 69. P. 311–325. doi: 10.1016/j.aquatox.2004.06.003

Perić L., Nerlović V., Žurga P., Žilić L., Ramšak A. Variations of biomarkers response in mussels Mytilus galloprovincialis to low, moderate and high concentrations of organic chemicals and metals // Chemosphere. 2017. Vol. 174. P. 554–562. doi: 10.1016/j.chemosphere.2017.01.138

Radenac G., Miramand P., Tardy J. Search for impact of a dredged material disposal site on growth and metal contamination of Mytilus edulis L. in Charente Maritime (France) // Mar. Pollut. Bull. 1997. Vol. 34. P. 721–729. doi: 10.1016/S0025-326X(97)00011-8

Rao D. G. V. P., Khan M. A. Q. Zebra mussels: Enhancement

of copper toxicity by high temperature and its relationship with respiration and metabolism // Water Environ. Res. 2000. Vol. 72. P. 175–178. doi: 10.2175/106143000X137257

Regoli F., Giuliani M. E. Oxidative pathways of chemical toxicity and oxidative stress biomarkers in marine organisms // Mar. Environ. Res. 2014. Vol. 93. P. 106–117. doi: 10.1016/j.marenvres.2013.07.006

Regoli F., Principato G. Glutathione, Glutathione-Dependant and Antioxidant Enzymes in Mussel, Mytilus Gallopro Vincilis Exposed to Metals in Different Field and Laboratory Condition. Implications for a Proper Use of Biochemical Markers // Aquatic Toxicology. 1995. Vol. 31. P. 143–164. doi: 10.1016/0166-445X(94)00064‑W

Sinicropi M. S., Amantea D., Caruso A., Saturnino C. Chemical and biological properties of toxic metals and use of chelating agents for the pharmacological treatment of metal poisoning // Arch. Toxicol. 2010. Vol. 84, no. 7. P. 501–520. doi: 10.1007/s00204‑010‑0544‑6

Syversen T., Kaur P. The toxicology of mercury and its compounds // J. Trace Elem. Med. Biol. 2012. Vol. 26, no. 4. P. 215–226. doi: 10.1016/j.jtemb.2012.02.004

Sze P. W. C., Lee S. Y. Effects of chronic copper exposure on the green mussel Perna viridis // Mar. Biol. 2000. Vol. 137. P. 379–392. doi: 10.1007/s002270000350

Sze P. W. C., Lee S. Y. The potential role of mucus in the depuration of copper from the mussels Perna viridis (L.) and Septifer virgatus (Wiegmann) // Marine Pollution Bull. 1995. Vol. 31, no. 4–12. P. 390–393. doi: 10.1016/0025-326X(95)00140‑I

Vega-López A., Ayala-López G., Posadas-Espadas B. P., Olivares-Rubio H. F., Dzul-Caamal R. Relations of oxidative stress in freshwater phytoplankton with heavy metals and polycyclic aromatic hydrocarbons // Comp. Biochem. Physiol. A Mol. Integr. Physiol. 2013. Vol. 165, no. 4. P. 498–507. doi: 10.1016/j.cbpa.2013.01.026

Viarengo A., Mancinelli G., Pertica M., Fabbri R., Orunesu M. Effects of heavy metals on the Ca (2+) – ATPase activity present in gill cell plasma-membrane of mussels (Mytilus galloprovincialis Lam.) // Comp. Biochem. Physiol. C. 1993. Vol. 106, no. 3. P. 655–660. doi: 10.1016/0742-8413(93)90223-8

Viarengo A., Pertica M., Mancinelli G., Burlando B., Canesi L., Orunesu M. In vivo effects of copper on the calcium homeostasis mechanisms of mussel gill cell plasma membranes // Comp. Biochem. Physt. C. 1996. Vol. 113. P. 421–425. doi: 10.1016/0742-8413(96)00004-7

Vosloo D., Sara J., Vosloo A. Acute responses of brown mussel (Perna perna) exposed to sub-lethal copper levels: integration of physiological and cellular responses // Aquat. Toxicol. 2012. Vol. 15, no. 106–107. P. 1–8. doi: 10.1016/j.aquatox.2011.10.001

Wang N., Ingersoll C. G., Greer I. E., Hardesty D. K., Ivey C. D., Kunz J. L., Brumbaugh W. G., Dwyer F. J., Roberts A. D., Augspurger T., Kane C. M., Neves R. J., Barnhart M. C. Chronic toxicity of copper and ammonia to juvenile freshwater mussels (Unionidae) // Environ. Toxicol. Chem. 2007. Vol. 26. P. 2048–2056. doi: 10.1897/06-524R.1

Wang W.‑X., Rainbow P. S. Significance of metallothioneins in metal accumulation kinetics in marine animals // Comp. Biochem. Physiol. Part C: Toxicol. Pharmacol. 2010. Vol. 152. P. 1–8.

Wang W. X., Pan K., Tan Q. G., Guo L., Simpson C. L. Estuarine pollution of metals in China: science and mitigation // Environ. Sci. Technol. 2014. Vol. 48. P. 9975–9976.

Yan B., Wang L., Li Y., Liu N., Wang Q. Effects of cadmium on hepatopancreatic antioxidant enzyme activity in freshwater crab (Sinopotamon yangtsekiense) // Acta Zool. Sin. 2007. Vol. 53(6). P. 1121–1128.

Zorita I., Bilbao E., Schad A., Cancio I., Soto M., Cajaraville M. P. Tissue- and cell-specific expression of metallothionein genes in cadmium- and copper-exposed mussels analyzed by in situ hybridization and RTPCR // Toxicol. Appl. Pharmacol. 2007. Vol. 220, no. 2. P. 186–196.

References in English

Chuiko G. M. Biomarkery v gidroekotoksikologii: printsipy, metody i metodologiya, praktika ispol’zovaniya [Biomarkers in hydroecotoxicology: principles, methods and methodology, practice of use]. Ekol. monitoring. Ch. VIII. Sovr. probl. monitoringa presnovodnykh ekosistem: ucheb. posobie [Ecol. monitoring. Part VIII. Current probl. of monitoring freshwater ecosystems: A Study guide]. Nizhnii Novgorod: NNGU, 2014.

P. 309–326.

Dauval’ter V. A., Kashulin N. A. Izmenenie kontsentratsii nikelya i medi v poverkhnostnykh sloyakh donnykh otlozhenii oz. Imandra za poslednie polveka [Changes in concentrations of nickel and copper in the surface layers of sediments of Lake Imandra for the last half century]. Vestnik MGTU [Vestnik MSTU]. 2015. Vol. 18, no. 2. P. 307–321.

Gosudarstvennyi doklad “O sostoyanii i ispol’zovanii vodnykh resursov Rossiiskoi Federatsii v 2017 godu” [State report ‘On the state and use of water resources of the Russian Federation in 2017’]. Moscow: NIA-Priroda, 2018. 298 p.

Luk’yanova O. N. Molekulyarnye biomarkery [Molecular biomarkers]. Vladivostok: DVGAEU, 2001. 196 p.

Mishukova T. G., Osipov A. A., Sal’nikov I. A. Opredelenie soderzhaniya mikroelementov v pit’evykh vodakh Orenburgskoi oblasti [Determination of trace elements in drinking waters of the Orenburg Region]. Vestnik Orenburgskogo gos. univ. [Proceed. Orenburg St. Univ.]. 2015. Vol. 10, no. 185. P. 303–307.

Moiseenko T. I., Kudryavtseva L. N., Gashkina N. A. Rasseyannye elementy v poverkhnostnykh vodakh sushi: tekhnofil’nost’, bioakkumulyatsiya i ekotoksikologiya [Scattered elements in surface land waters: Technophilicity, bioaccumulation, and ecotoxicology]. Moscow: Nauka, 2006. 261 p.

Ozera Karelii: Spravochnik [Lakes of Karelia: A Reference book]. Petrozavodsk: KarRC RAS, 2013. 464 p.

Prikaz ot 13 dekabrya 2016 goda N 552 “Ob utverzhdenii

normativov kachestva vody vodnykh ob’ektov rybokhozyaistvennogo znacheniya, v tom chisle normativov

predel’no dopustimykh kontsentratsii vrednykh veshchestv v vodakh vodnykh ob’ektov rybokhozyaistvennogo znacheniya (s izmeneniyami na 12 oktyabrya 2018 goda)” [Order No. 552 of December 13, 2016 ‘On approval of water quality standards for fishery water bodies, including maximum permissible concentrations of harmful substances in the waters of fishery water bodies (as amended on October 12, 2018) ’. Ministry of Agriculture of the Russian Federation. Elektronnyi fond pravovoi

inormativno-tekhnicheskoi dokumentatsii [Electronic fund of legal and regulatory technical documentation]. URL: http://docs.cntd.ru/document/420389120 (accessed: 15.11.2019).

Slukovskii Z. I. Normirovanie po litiyu kontsentratsii tyazhelykh metallov v donnykh otlozheniyakh ozer Ladozhskoe i Chetyrekhverstnoe (Respublika Kareliya) [Normalization of the concentrations of heavy metals with respect to lithium in bottom sediments of Lakes Ladoga and Chetyrekhverstnoye (Republic of Karelia)]. Khimiya v interesakh ustoichivogo razvitiya [Chemistry for Sustainable Development]. 2015. Vol. 23, no. 4. P. 397–408. doi: 10.15372/KhUR20150409

Slukovskii Z. I., Polyakova T. N. Analiz nakopleniya tyazhelykh metallov v organizme oligokhet iz rechnykh donnykh otlozhenii urbanizirovannoi sredy [Analysis of accumulation of heavy metals from river bottom sediments of the urban environment in the bodies of oligochaetes]. Biol. vnutr. vod [Inland Water Biol.]. 2017. Vol. 3. P. 73–82. doi: 10.7868/S032096521703010X

Sukhovskaya I. V., Borvinskaya E. V., Smirnov L. P., Nemova N. N. Sravnitel’nyi analiz metodov opredeleniya kontsentratsii belka – spektrofotometrii v diapazone 200–220 nm i po Bredford [Comparative analysis of the methods for determining protein concentration – spectrophotometry in the 200–220 nm range and the Bradford protein assay]. Trudy KarNTs RAN

[Trans. KarRC RAS]. 2010. Vol. 2. P. 68–71.

Shilova N. A. Vliyanie tyazhelykh metallov na predstavitelei

presnovodnogo fito- i zooplanktona v usloviyakh zasoleniya [Effect of metal concentration on freshwater phyto – and zooplankton under salinization conditions]: Summary of PhD (Cand. of Biol.) thesis. Saratov, 2014. 133 p.

Ahsanullah M., Williams A. R. Sublethal effects and bioaccumulation of cadmium, chromium, copper and zinc in the marine amphipod Allorchestes compressa. Mar. Biol. 1991. Vol. 108. P. 59–65. doi: 10.1007/BF01313471

Beers R. F. Jr., Sizer I. W. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. Biol. Chem. 1952. Vol. 195, no. 1. P. 133–140.

Cajaraville M. P., Bebianno M. J., Blasco J., Porte C., Sarasquete C., Viarengo A. The use of biomarkers to assess

the impact of pollution in coastal environments of the Iberian Peninsula: a practical approach. Sci. Total. Environ. 2000. Vol. 247, no. 2–3. P. 295–311. doi: 10.1016/s0048-9697(99)00499-4

Canesi L., Ciacci C., Fabbri R., Marcomini A., Pojana G., Gallo G. Bivalve mollusks as a unique target group for nanoparticle toxicity. Mar. Environ. Res. 2012. Vol. 76. P. 16–21. doi: 10.1016/j.marenvres.2011.06.005

Canesi L., Malatesta M., Battistelli S., Gallo G., Gazzanelli G. Identification of epidermal growth factor receptors by immunoelectron microscopy in isolated mussel digestive gland cells. Abstracts 19th Conference of European Comparative Endocrinologists (Nijmegen, The Netherlands, September 1–5, 1998).

Chapman P. M. Environmental risks of inorganic metals and metalloids: A continuing, evolving scientific odyssey. Human Ecol. Risk Assess. 2008. Vol. 14, no. 1. P. 5–40. doi: 10.1080/10807030701790272

Conradi M., Depledge M. H. Population responses of the marine amphipod Corophiumvolutator (Pallas, 1766) to copper. Aquat. Toxicol. 1998. Vol. 44. P. 31–45. doi: 10.1016/S0166-445X(98)00069-1

Cosson R. P., Thiébaut E., Company R., Castrec-Rouelle M., Colaço A., Martins I., Sarradin P. M., Bebianno M. J. Spatial variation of metal bioaccumulation in the hydrothermal vent mussel Bathymodiolus azoricus. Mar. Environ. Res. 2008. Vol. 65, no. 5. P. 405–15. doi: 10.1016/j.marenvres.2008.01.005

Directive 2008/105/EC of the European Parliament and of the Council of 16 December 2008 on environmental quality standards in the field of water policy, amending and subsequently repealing Council Directives 82/176/EEC, 83/513/EEC, 84/156/EEC, 84/491/EEC, 86/280/EEC and amending Directive 2000/60/EC of the European Parliament and of the Council. Official J. of the European Union. 2008. Vol. L 348. P. 84–97.

Dondero F., Piacentini L., Banni M., Rebelo M., Burlando B., Viarengo A. Quantitative PCR analysis of two molluscan metallothionein genes unveils differential expression and regulation. Gene. 2005. Vol. 345, no. 2. P. 259–270. doi: 10.1016/j.gene.2004.11.031

Duffus J. H. “Heavy metals” a meaningless term? (IUPAC Technical Report). Pure Appl. Chem. 2002. Vol. 74, no. 5. P. 793–807.

Foster-Smith R. L. The effect of concentration of suspension of the filtration rates and pseudofaecal production for Mytilus edulis L., Cerastoderma edule (L.) and Venerupis pullastra (Montagu). J. Exp. Mar. Biol. Ecol. 1975. Vol. 17. P. 1–22. doi: 10.1016/0022-0981(75)90075

George S. G., Coombs T. L., Pirie B. J. S. Characterization of metal-containing granules from the kidney of the common mussel, Mytilus edulis. Biochim. Biophys. Acta (BBA) – General Subjects. 1982. Vol. 716, no. 1. P. 61–71. doi: 10.1016/0304-4165(82)90203-3

George S. G., Pirie B. J. S., Coombs T. L. The kinetics of accumulation and excretion of ferric hydroxide in Mytilus edulis (L.) and its distribution in the tissues. J. Exp. Mar. Biol. Ecol. 1976. Vol. 23, no. 1. P. 71–84. doi: 10.1016/0022-0981(76)90086-1

Geret F., Serafim A., Barreira L., Bebianno M. J. Effects of cadmium on anti-oxidant enzyme activities and lipid peroxidation in the gills of the clam, Ruditapes decussates. Biomarkers. 2002. Vol. 7, no. 3. P. 242–256.

Guéguen M., Amiard J. C., Arnich N., Badot P. M., Claisse D., Guérin T., Vernoux J. P. Shellfish and residual chemical contaminants: hazards, monitoring, and health risk assessment along French coasts. Rev. Environ. Contam. Toxicol. 2011. Vol. 213. P. 55–111. doi: 10.1007/978‑1‑4419‑9860‑6_3

Habig W. H., Pabst M. J., Jakoby W. B. Glutathione S-Transferases. The first enzymatic step in mercapturic acid formation. J. Biol. Chem. 1974. Vol. 249, no. 22. P. 7130–7139.

Hissin P. J., Hilf R. A fluorometric method for determination of oxidized and reduced glutathione in tissues. Anal. Biochem. 1976. Vol. 74, no. 1. P. 214–226.

Hoarau P., Damiens G., Roméo M., Gnassia-Barelli M., Bebianno M. J. Cloningand expression of a GSTpi gene in Mytilus galloprovincialis. Attempt to use the GST-pi transcript as a biomarker of pollution. Comp. Biochem. Physiol. 2006. Vol. 143. P. 196–203.

Huggett R. J., Kimerle R. A., Mehrle P. M., Bergman H. L. Biomarkers: Biochemical, physiological, and histological markers of anthropogenic stress. FL, BocaRaton: Lewis Publ., 1992.

Klaassen C. D., Liu J., Choudhuri S. Metallothionein: An intracellular protein to protect against cadmium toxicity. Annu. Rev. Pharmacol. Toxicol. 1999. Vol. 39. P. 267–294.

Larsen B. K., Pörtner H. O., Jensen F. B. Extraand intracellular acid-base balance and ionic regulation in cod (Gadusmorhua) during combined and isolated exposures to hypercapnia and copper. Mar. Biol. 1997. Vol. 128. P. 337–346. doi: 10.1007/s002270050099

Lyons B. P., Thain J. E., Stentiford G. D., Hylland K., Davies I. M., Vethaak A. D. Using biological effects tools to define Good Environmental Status under the European Union Marine Strategy Framework Directive. Mar. Pollut. Bull. 2010. Vol. 60, no. 10. P. 1647–51. doi: 10.1016/j.marpolbul.2010.06.005

Lysenko L., Sukhovskaya I., Borvinskaya E., Krupnova M., Kantserova N., Bakhmet I., Nemova N. Detoxification and protein quality control markers in the mussel Mytilus edulis (Linnaeus) exposed to crude oil: Salinity-induced modulation. Estuar. Coast. Shelf Sci. 2015. Vol. 167. P. 220–227.

March F. A., Dwyer F. J., Augspurger T., Ingersoll C. G., Wang N., Mebane C. A. An evaluation of freshwater mussel toxicity data in the derivation of water quality guidance and standards for copper. Environ. Toxicol. Chem. 2007. Vol. 26. P. 2066–2074. doi: 10.1897/06-560R.1

Marigomez I., Soto M., Cajaraville M. P., Angulo E., Giamberini L. Cellular and subcellular distribution of metals in molluscs. Microsc. Res. Tech. 2002. Vol. 56. P. 358–392. doi: 10.1002/jemt.10040

Noble J. E., Bailey M. A. Quantitation of protein. Methods Enzymol. 2009. Vol. 463. P. 73–95. doi: 10.1016/S0076-6879(09)63008-1

Parry H. E., Pipe R. K. Interactive effects of temperature and copper on immunocompetence and disease susceptibility in mussels (Mytilus edulis). Aquat. Toxicol. 2004. Vol. 69. P. 311–325. doi: 10.1016/j.aquatox.2004.06.003

Perić L., Nerlović V., Žurga P., Žilić L., Ramšak A. Variations of biomarkers response in mussels Mytilus galloprovincialis to low, moderate and high concentrations of organic chemicals and metals. Chemosphere. 2017. Vol. 174. P. 554–562. doi: 10.1016/j.chemosphere.2017.01.138

Radenac G., Miramand P., Tardy J. Search for impact of a dredged material disposal site on growth and metal contamination of Mytilus edulis L. in Charente Maritime (France). Mar. Pollut. Bull. 1997. Vol. 34. P. 721–729. doi:

1016/S0025-326X(97)00011-8

Rao D. G. V. P., Khan M. A. Q. Zebra mussels: Enhancement of copper toxicity by high temperature and its relationship with respiration and metabolism. Water Environ. Res. 2000. Vol. 72. P. 175–178. doi: 10.2175/106143000X137257

Regoli F., Giuliani M. E. Oxidative pathways of chemical toxicity and oxidative stress biomarkers in marine organisms. Mar. Environ. Res. 2014. Vol. 93. P. 106–117. doi: 10.1016/j.marenvres.2013.07.006

Regoli F., Principato G. Glutathione, Glutathione-Dependant and Antioxidant Enzymes in Mussel, Mytilus Gallopro Vincilis Exposed to Metals in Different Field and Laboratory Condition. Implications for a Proper Use of Biochemical Markers. Aquatic Toxicology. 1995. Vol. 31. P. 143–164. doi: 10.1016/0166-445X(94)00064‑W

Sinicropi M. S., Amantea D., Caruso A., Saturnino C. Chemical and biological properties of toxic metals and use of chelating agents for the pharmacological treatment of metal poisoning. Arch. Toxicol. 2010. Vol. 84, no. 7. P. 501–20. doi: 10.1007/s00204‑010‑0544‑6

Syversen T., Kaur P. The toxicology of mercury and its compounds. J. Trace Elem. Med. Biol. 2012. Vol. 26, no. 4. P. 215–26. doi: 10.1016/j.jtemb.2012.02.004

Sze P. W. C., Lee S. Y. Effects of chronic copper exposure on the green mussel Perna viridis.Mar. Biol. 2000. Vol. 137. P. 379–392. doi: 10.1007/s002270000350

Sze P. W. C., Lee S. Y. The potential role of mucus in the depuration of copper from the mussels Perna viridis (L.) and Septifer virgatus (Wiegmann). Marine Pollution Bull. 1995. Vol. 31, no. 4–12. P. 390–393. doi: 10.1016/0025-326X(95) 00140‑I

Vega-López A., Ayala-López G., Posadas-Espadas B. P., Olivares-Rubio H. F., Dzul-Caamal R. Relations of oxidative stress in freshwater phytoplankton with heavy metals and polycyclic aromatic hydrocarbons. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 2013. Vol. 165, no. 4. P. 498–507. doi: 10.1016/j.cbpa.2013.01.026

Viarengo A., Mancinelli G., Pertica M., Fabbri R., Orunesu M. Effects of heavy metals on the Ca (2+) – ATPase activity present in gill cell plasma-membrane of mussels (Mytilus galloprovincialis Lam.). Comp. Biochem. Physiol. C Toxicol. Pharmacol. 1993. Vol. 106, no. 3. P. 655–660. doi: 10.1016/0742-8413(93)90223-8

Viarengo A., Pertica M., Mancinelli G., Burlando B., Canesi L., Orunesu M. In vivo effects of copper on the calcium homeostasis mechanisms of mussel gill cell plasma membranes. Comp. Biochem. Physiol. C Toxicol. Pharmacol. 1996. Vol. 113. P. 421–425. doi: 10.1016/0742-8413(96)00004-7

Vosloo D., Sara J., Vosloo A. Acute responses of brown mussel (Perna perna) exposed to sub-lethal copper levels: integration of physiological and cellular responses. Aquat. Toxicol. 2012. Vol. 15, no. 106–107. P. 1–8. doi: 10.1016/j.aquatox.2011.10.001

Wang N., Ingersoll C. G., Greer I. E., Hardesty D. K., Ivey C. D., Kunz J. L., Brumbaugh W. G., Dwyer F. J., Roberts A. D., Augspurger T., Kane C. M., Neves R. J., Barnhart M. C. Chronic toxicity of copper and ammonia to juvenile freshwater mussels (Unionidae). Environ. Toxicol. Chem. 2007. Vol. 26. P. 2048–2056. doi: 10.1897/06-524R.1

Wang W. X., Pan K., Tan Q. G., Guo L., Simpson C. L. Estuarine pollution of metals in China: science and mitigation. Environ. Sci. Technol. 2014. Vol. 48. P. 9975–9976.

Wang W.‑X., Rainbow P. S. Significance of metallothioneins in metal accumulation kinetics in marine animals. Comp. Biochem. Physiol. Part C: Toxicol. Pharmacol. 2010. Vol. 152. P. 1–8.

Yan B., Wang L., Li Y., Liu N., Wang Q. Effects of cadmium on hepatopancreatic antioxidant enzyme activity in freshwater crab (Sinopotamon yangtsekiense). Acta Zool. Sin. 2007. Vol. 53(6). P. 1121–1128.

Zorita I., Bilbao E., Schad A., Cancio I., Soto M., Cajaraville M. P. Tissue- and cell-specific expression of metallothionein genes in cadmium- and copper-exposed mussels analyzed by in situ hybridization and RTPCR. Toxicol. Appl. Pharmacol. 2007. Vol. 220, no. 2. P. 186–196.




DOI: http://dx.doi.org/10.17076/eb1157

Ссылки

  • На текущий момент ссылки отсутствуют.


© Труды КарНЦ РАН, 2014-2019