ОЦЕНКА ЛИПИДНОГО СОСТАВА И НЕКОТОРЫХ КОМПОНЕНТОВ АНТИОКСИДАНТНОЙ СИСТЕМЫ МИДИЙ MYTILUS EDULIS В УСЛОВИЯХ ЭКСПЕРИМЕНТАЛЬНОГО ЗАРАЖЕНИЯ МЕТАЦЕРКАРИЯМИ HIMASTHLA ELONGATA

Наталья Николаевна Фокина, Ирина Викторовна Суховская, Татьяна Рудольфовна Руоколайнен, Альбина Александровна Кочнева, Игорь Николаевич Бахмет, Кирилл Евгеньевич Николаев, Иван Андреевич Левакин, Natalia Fokina, Irina Sukhovskaya, Tatiana Ruokolainen, Albina Kochneva, Igor Bakhmet, Kirill Nikolaev, Ivan Levakin

Аннотация


Проведено исследование длительного эффекта экспериментального заражения метацеркариями Himasthla elongata (Mehlis, 1831) (Himasthlidae) на некоторые биохимические показатели второго промежуточного хозяина - мидии Mytilus edulis (содержание восстановленной формы глутатиона, структурных и запасных липидов, жирных кислот общих липидов, а также активность ферментов глутатион-S-трансферазы, каталазы и пероксидазы). Показано, что паразитарная инвазия оказывает влияние на активность каталазы в жабрах и ноге у мидий, а также на содержание некоторых фосфолипидов и жирных кислот преимущественно в их жабрах, гепатопанкреасе и ноге. В ноге инвазированных мидий пониженная активность каталазы и повышенное содержание лизофосфатидилхолина и сфингомиелина, а также низкий уровень полиненасыщенных жирных кислот (преимущественно докозагексаеновой 22:6n-3 кислоты) отражают, главным образом присутствие метацеркарий H. elongata в исследуемом органе. В тоже время изменения некоторых биохимических показателей в жабрах у инвазированных мидий (главным образом, повышенная активность каталазы, сниженное содержание эфиров холестерина и модификации на уровне жирнокислотного спектра) указывают на наличие стрессового воздействия, оказываемого метацеркариями H. elongata на моллюсков. Предполагается, что исследуемые биохимические показатели подвергаются значительному воздействию непосредственно в момент внедрения паразитов в ткани хозяина (в течение первых суток), тогда как длительный эффект нахождения паразита в организме второго промежуточного хозяина (на протяжении года) приводит к стабилизации в системе паразит-хозяин, в том числе на уровне исследуемых биохимических показателей. Данное предположение требует проведения дополнительных исследований.

Ключевые слова


фосфолипиды; жирные кислоты; восстановленный глутатион; каталаза; глутатион-S-трансфераза; двустворчатые моллюски; трематоды

Полный текст:

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Литература


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DOI: http://dx.doi.org/10.17076/eb731

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